KMID : 0364820130490040369
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Korean Journal of Microbiology 2013 Volume.49 No. 4 p.369 ~ p.376
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Conversion of Ginsenoside Rb1 and Taxonomical Characterization of Stenotrophomonas sp. 4KR4 from Ginseng Rhizosphere Soil
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Jeon In-Hwa
Cho Geon-Yeong Han Song-Ih Yoo Sun-Kyun Whang Kyung-Sook
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Abstract
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We isolated the ¥â-glucosidase producing bacteria (BGB) in ginseng root system (rhizosphere soil, rhizoplane, inside
of root). Phylogenetic analysis of the 28 BGB based on the 16S rRNA gene sequences, BGB from rhizosphere soil
belong to genus Stenotrophomonas (3 strains), Bacillus (1 strain), and Pseudoxanthomonas (1 strain). BGB isolates
from rhizoplane were Stenotrophomonas (16 strains), Streptomyces (1 strain) and Microbacterium (1 strain). BGB
from inside of root were categorized into Stenotrophomonas (3 strains) and Lysobacter (2 strains). Especially,
Stenotrophomonas comprised the largest portion (approximately 90%) of total isolates and Stenotrophomonas was a
dominant group of the ¥â-glucosidase producing bacteria. We selected strain 4KR4, which had high ¥â-glucosidase
activity (108.17 unit), could transform ginsenoside Rb1 into Rd, Rg3, and Rh2 ginsenosides. In determining its relationship on the basis of 16S rRNA sequence, 4KR4 strain was most closely related to Stenotrophomonas rhizophila e-p10T (AJ293463) (99.62%). Therefore, on the basis of these polyphasic taxonomic evidence, the ginsenoside Rb1 converting bacteria 4KR4 was identified as Stenotrophomonas sp. 4KR4 (=KACC 17635).
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KEYWORD
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Stenotrophomonas sp., ginsenoside Rb1, ginsenoside Rh2, Ginseng soil
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